General Cell Based Assays
SAPPHIRE NORTH AMERICA NaveniFlex Tissue MR Atto647N
NaveniFlex Tissue MR Atto647N is designed specifically for the detection of proteins, protein interactions or modification in tissues. It improves sensitivity and removes high background seen in certain samples. NaveniFlex Tissue MR Atto647N is compatible with a mouse and rabbit primary antibody pair and uses a Atto647N fluorophore for detection.
Enzo Life Sciences Red hydrogen peroxide assay kit (1Kit)
Red Hydrogen Peroxide Assay Kit provides a simple absorbance-based or fluorometric assay to detect and quantify hydrogen peroxide or peroxidase activity in biochemical assays, cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized ‘mix and read’ assay that is suitable for both 96- and 348-well formats and is compatible with HTS liquid handling. Applications: Fluorescence microscopy, HTS .
Abcam TET Hydroxylase Activity Quantification Kit (Fluorometric)
TET Hydroxylase Activity Quantification Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress mINSRb Functional Assay, 2-Plate Kit. (93-1017E3CP16M)
The PathHunter eXpress mINSRb Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated mINSRb dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged mINSRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress P2RY1 Activated GPCR Internalization Assay, 2pl Kit. (93-0967E3CP0M)
The PathHunter P2RY1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of P2RY1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged P2RY1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting P2RY1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL5RA/CSF2RB Dimerization Assay, 10pl Kit. (93-0972E3CP0L)
The PathHunter IL5RA/CSF2RB Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-5) induced dimerization of IL5RA/CSF2RB receptors. This product uses EFC technology. The cell line included overexpresses CSF2RB target protein fused to PK alongside IL5RA target protein fused to EA. Upon ligand-induced dimerization of IL5RA/CSF2RB, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mGCGR CHO-K1 β-Arrestin-1 GPCR Assay, 10pl Kit. (93-0973E2MCP2L)
The PathHunter mGCGR (ARR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. glucagon) based activation of mGCGR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGCGR and EA-tagged β-Arrestin-1. Activation of mGCGR stimulates the recruitment of β-Arrestin-1 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter OSMRb/IL31R Bioassay Kit, 10-Plate. (93-1002Y3-00084)
The PathHunter OSMRb/IL31R Dimerization Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-31) induced dimerization of OSMRb/IL31R receptors. This product uses EFC technology. The cell line included overexpresses OSMRb target protein fused to PK alongside IL31RA target protein fused to EA. Upon ligand-induced dimerization of OSMRb/IL31R, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress mGCGR CHO-K1 β-Arrestin-1 GPCR Assay, 2-Plate Kit. (93-0973E2MCP2M)
The PathHunter mGCGR (ARR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. glucagon) based activation of mGCGR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGCGR and EA-tagged β-Arrestin-1. Activation of mGCGR stimulates the recruitment of β-Arrestin-1 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TYK2 Activity Assay, 10-Plate Kit. (93-0875E3CP16L)
The PathHunter eXpress TYK2 Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting the TYK kinase domain. The cell line is engineered to express the catalytic domain from TYK2 fused to the cytokine receptor binding domain (CRBD) of JAK2. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the GHR-PK. Activation of the GHR-PK induces receptor phosphorylation by the TYK2 catalytic domain. This leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CXCR7 Activated GPCR Internalization Assay, 10pl Kit. (93-0738E3CP27L)
The PathHunter CXCR7 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CXCR7 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CXCR7. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CXCR7-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OPN5 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0704E2CP1L)
The PathHunter OPN5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of OPN5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OPN5 and EA-tagged β-Arrestin-2. Activation of OPN5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CXCR6 Total Internalization Assay, 10pl Kit. (93-0886E3CP0L)
The PathHunter CXCR6 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CXCR6 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged CXCR6 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CXCR6 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress XBP1 Nuclear Translocation Assay, 10pl Kit. (93-0857E3CP5L)
The PathHunter eXpress XBP1 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the IRE1/XBP1 pathway. This kit assesses ligand (e.g. thapsigargin) based pathway activation via detection of resulting XBP1 nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged XBP1 and nuclear localized EA. Upon pathway activation, induced nuclear translocation of XBP1 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.